Description
Usage Instructions for Vet Expert Rapid FIV Ab/FeLV Ag Test Kit
Basic Principles
The Vet Expert FIV Ab/FeLV Ag test is a chromatographic immunoassay for the qualitative detection of antibodies against the Feline Immunodeficiency Virus (FIV) and antigens of the Feline Leukemia Virus (FeLV) in feline serum, plasma, or whole blood. The letters T and C on the test plate indicate the test line (T) and the control line (C). The control line (C) serves as a procedural control and should always appear if the test is performed correctly and the reagents function properly. A test line (T) will appear in the results window if antibodies or antigens are present in the sample.
- Sensitivity: FIV 96.0% / FeLV 99.9%
- Specificity: FIV 98.0% / FeLV 99.9%
Materials Provided (Available in 2, 5, or 10 Test Kits)
- 2/5/10 Vet Expert FIV Ab/FeLV Ag test plates
- 1/2 diluent vials
- 2/5/10 anticoagulant vials
- 2/5/10 pipettes (10 µl)
- Instructions for use
Precautions
- For veterinary diagnostic use only.
- Strict adherence to instructions is necessary for accurate results.
- The test plate is sensitive to moisture and heat; perform the test immediately after opening.
- Use materials only once.
- Avoid touching the membrane in the result window.
- Do not use the test beyond the expiration date on the packaging.
- Do not use the test if the pouch is damaged.
- Do not mix materials from different Lot numbers.
- Handle samples as potentially infectious.
- Disinfect and dispose of all samples and materials according to local regulations.
- Consider test results invalid after the specified reading time.
Test Kit Storage
Store the test kit at 2 ~ 30 °C. DO NOT FREEZE. Protect from direct sunlight.
Sample Collection and Preparation
The test can be performed with serum, plasma, or whole blood, though serum is the preferred sample.
- Serum: Collect whole blood (without anticoagulant), allow it to clot for 30 minutes, then centrifuge to obtain serum.
- Plasma: Collect whole blood into a collection vial containing anticoagulants, then centrifuge to obtain plasma.
- Whole Blood: Collect blood into the provided anticoagulant vial (maximum volume 1.5 ml). If whole blood with anticoagulant is not tested immediately, refrigerate at 2~8°C and use within 24 hours.
Store serum samples at 2~8°C. For longer storage, freeze samples at -20°C or lower. Avoid repeated freeze-thaw cycles. Samples with sediment may yield unreliable results and should be clarified before testing. Hemolyzed or contaminated samples may produce incorrect results.
Test Procedure
- Bring all reagents to room temperature (15~30°C) before use.
- Remove the test from its foil pouch and place it on a flat, dry surface.
- Using the included pipette, add 10 µl of serum/plasma/whole blood to the sample well.
- Add 2 drops of diluent to the sample well.
- The sample will migrate across the results window. If migration does not start within 1 minute, add one more drop of diluent to the sample well.
- Read the result after 10 minutes.
Interpretation of Results
- Negative Result: Only the control line (C) appears in the results window.
- Positive Result: Both the test line (T) and control line (C) appear, indicating the presence of antigens/antibodies. Even a faint line is considered positive.
- Invalid Result: If the control line (C) does not appear, the result is invalid, and the sample should be retested.
Test Limitations
While the Vet Expert FIV Ab/FeLV Ag test is highly accurate, a small percentage of false results may occur. Additional clinical testing is recommended if results are inconclusive. As with all diagnostic tests, a definitive clinical diagnosis should not rely solely on a single test result but should be based on a comprehensive evaluation of clinical and laboratory findings.
