Description
Usage Instructions for Vet Expert Rapid FCoV Ab Test Kit
Basic Principles
The Vet Expert FCoV Ab test is a chromatographic immunoassay for the qualitative detection of coronavirus antibodies in feline serum, plasma, or whole blood. The T and C letters on the test plate indicate the test line (T) and the control line (C). The control line (C) serves as a procedural control and should always appear if the test is performed correctly. A test line (T) will be visible in the results window if antibodies are present in the sample.
- Sensitivity: 96.0%
- Specificity: 97.9%
Materials Provided (5 Tests per Kit)
- 5 Vet Expert FCoV Ab test plates
- 1 diluent vial
- 5 anticoagulant vials
- 5 pipettes (10 µl)
- Instructions for use
Precautions
- Veterinary diagnostic use only.
- Follow instructions carefully for accurate results.
- The test plate is sensitive to moisture and heat; use it immediately after opening.
- Single-use materials only.
- Avoid touching the membrane in the result window.
- Do not use the test after the expiration date on the packaging.
- Do not use the test if the pouch is damaged.
- Do not mix materials from different Lot numbers.
- Handle samples as potentially infectious.
- Disinfect and dispose of samples and materials according to local regulations.
- Consider results invalid after the specified reading time.
Storage
Store the test at 2 ~ 30 °C. DO NOT FREEZE. Keep away from direct sunlight.
Sample Collection and Preparation
The test can be performed with serum, plasma, or whole blood, though serum is the preferred sample.
- Serum: Collect whole blood (no anticoagulant), allow it to clot for 30 minutes, then centrifuge to obtain serum.
- Plasma: Collect whole blood into the provided collection vial (containing anticoagulants), then centrifuge to obtain plasma.
- Whole Blood: Collect blood into the provided anticoagulant vial (max. 1.5 ml). If whole blood is not tested immediately, refrigerate at 2~8°C and use within 24 hours.
Store serum samples at 2~8°C. For longer storage, freeze samples at -20°C or below. Avoid repeated freeze-thaw cycles. Samples with sediment should be clarified before testing. Hemolyzed or contaminated samples may yield incorrect results.
Test Procedure
- Allow all reagents to reach room temperature (15~30°C).
- Remove the test from its foil pouch and place on a flat, dry surface.
- Using a pipette (included), add 10 µl of whole blood to the sample well.
- Add 2 drops of diluent to the sample well.
- The sample will migrate across the results window. If migration does not begin within 1 minute, add another drop of diluent to the sample well.
- Read the result after 10 minutes. Do not read after 20 minutes.
Interpretation of Results
- Negative Result: Only the control line (C) appears in the results window.
- Positive Result: Both the test line (T) and the control line (C) appear, indicating the presence of antibodies. Even a faint line is considered positive.
- Invalid Result: If the control line (C) does not appear, the result is invalid, and the sample should be retested.
Test Limitations
While the Vet Expert FCoV Ab test is highly accurate, a small rate of false results may occur. Additional clinical testing is recommended if results are inconclusive. As with all diagnostic tests, a final clinical diagnosis should not rely on a single test result but should be based on a full clinical and laboratory evaluation.
